I did not use those parameters for Y axis on the X axis and the X axis did not even show while the Y axis still exist. Please look at my updated small code.
‘layout’:go.Layout(
width = 800,
height = 600,
title = ‘Well Plate’,
xaxis = {‘title’:‘Column’,
‘showgrid’:False},
yaxis = {‘title’:‘Row’,
‘showgrid’:False,
‘showline’:False,
‘zeroline’:False,
‘autorange’:‘reversed’})},