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2 different regression equations

I am getting two different regression equations for the exact same data. Can someone please explain why this may be the case?

Here is my data: https://plot.ly/~croth/35/

When I make a scatter plot with Col 1 (x) and Col 2 (y), and then fit a linear regression to it, I get a different equation than the line that I fit using Col 3 and Col 4, even though the ordered pairs are exactly the same. I thought maybe there was a typo in the data, but I have checked it many times.